HYSBYSIAD O GONTRACT - CENEDLAETHOL

1 Manylion yr Awdurdod

1.1

Enw a Chyfeiriad yr Awdurdod

1.2

Cyfeiriad ar gyfer cael dogfennaeth

1.3

Mae'n rhaid dychwelyd dogfennau wedi'u cwblhau at:

2 Manylion y Contract

2.1

Teitl

Knock-out Cell Line Generation

2.2

Disgrifiad o'r nwyddau neu'r gwasanaethau sydd eu hangen

The title of the wider research project is ‘Repeat-induced mutagenesis in Huntington’s disease’ and the overall aim is to understand how expanded CAG/CTG repeats can cause mutagenesis at the Huntington’s disease locus.

Huntington’s disease (HD) is a progressive neurodegenerative disease characterised by involuntary movements, apathy, and cognitive decline. Expansion of CAG/CTG repeats within the HTT gene cause HD. These repeats are genetically unstable, leading to dramatic changes in repeat size, biased towards expansion over an individual’s lifetime. Somatic expansion in affected tissues is thought to modify disease presentation and many genes identified in genome-wide association studies play a role in DNA repair and in repeat instability.

Repeat instability, defined as gain or loss of repeat units relies on DNA repair processes although the exact mechanisms are still being elucidated. Although several DNA repair components have been implicated in repeat instability, how they work together and how potential sequence context may influence the process is unclear.

Part of this project involves investigating the effects of genes associated with DNA repair on repeat instability. 10 different genes of interest have been identified and to investigate their effect on repeat instability, these genes need to be knocked-out in our custom HEK293 cell line.

The requirement is for separate knock-out of 10 genes in an immortalised cell line, GFP(CAG)101, which is HEK293-derived. Vincent Dion et al will provide the original cell line as starting material. This material will be mycoplasma tested before being sent.

2.3

Cod a Dosbarthiad yr Hysbysiad

2.4

Cyfanswm maint neu gwmpas y tendr

The requirement is for separate knock-out of 10 genes in an immortalised cell line, GFP(CAG)101, which is HEK293-derived. Vincent Dion et al will provide the original cell line as starting material. This material will be mycoplasma tested before being sent.

Further details can be found in Schedule 2 and 3.

3 Amodau ar gyfer Cymryd Rhan

3.1

Safonau gofynnol a'r cymhwyster sydd ei angen

Supplier has supplied at least 10 cell lines within the UK or Europe in the last 2 years. This must be evidenced as part of this tender.

4 Gwybodaeth Weinyddol

4.1

Math o Weithdrefn

Un cam

4.2

Rhif cyfeirnod a roddwyd i'r hysbysiad gan yr awdurdod contractio

CU.1555.RR

4.3

Terfynau Amser

4.5

Yr iaith neu'r ieithoedd y gellir ei/eu defnyddio i lunio tendrau neu geisiadau i gymryd rhan

4.6

Blwch Postio Cyflwyno Tendrau

5 Gwybodaeth Arall

5.1

Gwybodaeth Ychwanegol

To express interest, please use the following link and join the Tender Project via the tendering portal.

https://in-tendhost.co.uk/cardiffuniversity/aspx/Home

(WA Ref:138089)

5.2

Dogfennaeth Ychwanegol

5.3

Dyddiad cyhoeddi'r hysbysiad hwn